| | Verein | Trainer | Liga | Stadion | Sitz | Gegründet |
 | Louisville City | John Hackworth | USL Championship | Louisville Slugger Field | Louisville | 2014 |
| Hartford Athletic | | USL Championship | Dillon Stadium | Hartford | 2018 |
| Atlanta SC | Steve Guppy | | Atlanta Silverbacks Park | Atlanta | 1995 |
| Colorado Springs Switchbacks | | USL Championship | Sand Creek Stadium | Colorado Springs | 2013 |
| Richmond Kickers | Leigh Cowlishaw | USL League One | City Stadium | Richmond | 1993 |
| Forward Madison | | USL League One | Breese Stevens Field | Madison | 2018 |
| Rochester Rhinos | | | Sahlen's Stadium | Rochester | 1996 |
| RGV FC Toros | | USL Championship | RGVFC Stadium | Edinburg | 2015 |
| Wilmington Hammerheads | | | Legion Stadium | Wilmington | 1996 |
| Penn FC | | | FNB Field | Harrisburg | 2003 |
| Chattanooga Red Wolves | | USL League One | David Stanton Field | Chattanooga | 2018 |
| Detroit City | Trevor James | USL League One | Keyworth Stadium | Detroit | 2012 |
| Rhode Island | | USL Championship | Beirne Stadium | Pawtucket | 2019 |
| San Antonio Scorpions | | | Heroes Stadium | San Antonio | 2011 |
| Miami United | Gerardo Reinoso | National Premier Soccer League | Flamingo Park | Miami | 2012 |
| Union Omaha | | USL League One | Werner Park | Omaha, Nebraska | 2019 |
| Tormenta FC | Ian Cameron | USL League One | Erk Russell Park | Statesboro | 2015 |
| Jacksonville Armada | | National Premier Soccer League | Baseball Grounds of Jacksonville | Jacksonville | 2013 |
| Chattanooga FC | | | Finley Stadium | Chattanooga | 2009 |
| Loudoun United | | USL Championship | Loudoun United Stadium | Leesburg | 2018 |
| Central Valley Fuego | Jermaine Jones | USL League One | Parmer Field | Fresno, California | 2020 |
| Northern Colorado Hailstorm | | USL League One | Future Legends Complex | Windsor, Colorado | 2021 |
| Dayton Dutch Lions | Matt Weston | | Miami Valley South Stadium | Dayton | 2009 |
| Saint Louis FC | Steve Trittschuh | | St. Louis Soccer Park | St. Louis | 2014 |
| Lexington SC | | USL League One | Toyota Stadium | Lexington | 2021 |
| Spokane Velocity FC | | USL League One | Skyline Sports Complex | Spokane, Washington | 2021 |
| Charlotte Eagles | | | Charlotte Christian School | Charlotte | 1991 |
| Greenville Triumph SC | | USL League One | Legacy Early College | Greenville | 2018 |
| San Diego FC | Mikey Varas | USL League One | Snapdragon Stadium | San Diego | 2023 |
| California United | | | Titan Stadium | Fullerton | 2017 |
| San Diego Loyal | | USL Championship | Torero Stadium | San Diego | 2019 |
| One Knoxville SC | | USL League One | Highmark Stadium | Knoxville, Tennessee | 2021 |
| FC Tucson | | USL League One | Kino Sports Complex | Tucson | 2010 |
| Lansing Ignite | | | Cooley Law School Stadium | Lansing | 2018 |
| Rayo OKC | | | Miller Stadium | Oklahoma City | 2015 |
| Philadelphia Union-2 | | | Goodman Stadium | Bethlehem | 2015 |
| San Francisco Deltas | | | Kezar Stadium | San Francisco | 2016 |
| Austin Aztex | | | House Park | Austin | 2008 |
| Portland Timbers 2 | Cameron Knowles | | Merlo Field | Portland | 2015 |
| Long Island Rough Riders | | | Hofstra University Soccer Stadium | South Huntington | 1994 |
| Carolina Core FC | Donovan Ricketts | MLS Next Pro | Truist Point | High Point | 2022 |
| FC Baltimore Christos | | National Premier Soccer League | Marriotts Ridge High School | Marriottsville, Maryland | 2018 |
| Peachtree City MOBA | | | MOBA Soccer Stadium | Peachtree, Georgia | 2013 |
| Club de Lyon | Héctor Almandoz | NISA | Daytona Stadium | Orlando | 2021 |
| Atlanta United 2 | Steve Cooke | USL Championship | Coolray Field | Lawrenceville | 2017 |
| Los Angeles Force | Dekel Keinan | NISA | Veterans Memorial Stadium | Long Beach | 2019 |
| Sporting KC II | Benny Feilhaber | USL Championship | Children's Mercy Park | Kansas City | 2015 |
| Milwaukee Torrent | | National Premier Soccer League | Hart Park | Milwaukee | 2015 |
| FC Miami City | | | Central Broward Park | Miami | 2014 |
| Tacoma Defiance | | USL Championship | Cheney Stadium | Seattle | 2014 |
