| | Verein | Trainer | Liga | Stadion | Sitz | Gegründet |
 | Atlanta United | | Major League Soccer | Mercedes-Benz Stadium | Atlanta | 2014 |
| Nashville SC | B.J. Callaghan | Major League Soccer | Geodis Park | Nashville | 2016 |
| Real Salt Lake | Pablo Mastroeni | Major League Soccer | Rio Tinto Stadium | Salt Lake City | 2004 |
| St. Louis City SC | | Major League Soccer | St. Louis City Stadium | St. Louis | 2019 |
| Minnesota United | Eric Ramsay | Major League Soccer | TCF Bank Stadium | Minnesota | 2009 |
| Charlotte FC | Dean Smith | Major League Soccer | Bank of America Stadium | Charlotte | 2019 |
| Chivas USA | | | Dignity Health Sports Park | Los Angeles | 2004 |
| New York Cosmos | Alecko Eskandarian | National Premier Soccer League | James M. Shuart Stadium | New York | 1971 |
| Indy Eleven | Sean Mcauley | USL Championship | Michael A. Caroll Stadium | Indianapolis | 2013 |
| Phoenix Rising FC | | USL Championship | Phoenix Rising FC Soccer Complex | Peoria | 2014 |
| San Antonio FC | Alen Marcina | USL Championship | Toyota Field | San Antonio, Texas | 2015 |
| Inter Miami CF II | Federico Higuaín | USL League One | DRV PNK Stadium | Miami | 2019 |
| Orange County SC | | USL Championship | Titan Stadium | Fullerton | 2010 |
| Tampa Bay Rowdies | Robbie Neilson | USL Championship | Al Lang Stadium | Tampa | 2008 |
| Miami FC | | USL Championship | FIU Stadium | Miami | 2015 |
| Charlotte Independence | Mike Jeffries | USL Championship | American Legion Memorial Stadium | Charlotte | 2014 |
| Austin Bold | | USL Championship | Bold Stadium | Austin | 2017 |
| North Carolina FC | | USL League One | WakeMed Soccer Park | Cary | 2006 |
| Las Vegas Lights | | USL Championship | Cashman Field | Las Vegas | 2017 |
| Pittsburgh Riverhounds | Bob Lilley | USL Championship | Highmark Stadium | Pittsburgh | 1999 |
| Sacramento Republic FC | Mark Briggs | USL Championship | Charles C. Hughes Stadium | Sacramento | 2012 |
| Memphis 901 | Stephen Glass | USL Championship | AutoZone Park | Memphis | 2018 |
| Birmingham Legion | Tom Soehn | USL Championship | BBVA Compass Field | Birmingham | 2017 |
| OKC Energy FC | | USL Championship | Pribil Stadium | Oklahoma City | 2013 |
| Monterey Bay FC | | USL Championship | Freeman Stadium | Monterey County | 2021 |
| Oakland Roots | | USL Championship | Laney College | Oakland | 2018 |
| El Paso Locomotive | Wilmer Cabrera | USL Championship | Southwest University Park | El Paso | 2018 |
| New Mexico United | | USL Championship | Isotopes Park | Albuquerque | 2018 |
| FC Tulsa | | USL Championship | ONEOK Field | Tulsa | 2013 |
| Charleston Battery | John Wilson | USL Championship | Blackbaud Stadium | Charleston | 1993 |
| Louisville City | John Hackworth | USL Championship | Louisville Slugger Field | Louisville | 2014 |
| Hartford Athletic | | USL Championship | Dillon Stadium | Hartford | 2018 |
| Atlanta SC | Steve Guppy | | Atlanta Silverbacks Park | Atlanta | 1995 |
| Colorado Springs Switchbacks | | USL Championship | Sand Creek Stadium | Colorado Springs | 2013 |
| Richmond Kickers | Leigh Cowlishaw | USL League One | City Stadium | Richmond | 1993 |
| Forward Madison | | USL League One | Breese Stevens Field | Madison | 2018 |
| Rochester Rhinos | | | Sahlen's Stadium | Rochester | 1996 |
| RGV FC Toros | | USL Championship | RGVFC Stadium | Edinburg | 2015 |
| Wilmington Hammerheads | | | Legion Stadium | Wilmington | 1996 |
| Penn FC | | | FNB Field | Harrisburg | 2003 |
| Chattanooga Red Wolves | | USL League One | David Stanton Field | Chattanooga | 2018 |
| Detroit City | Trevor James | USL League One | Keyworth Stadium | Detroit | 2012 |
| Rhode Island | | USL Championship | Beirne Stadium | Pawtucket | 2019 |
| San Antonio Scorpions | | | Heroes Stadium | San Antonio | 2011 |
| Miami United | Gerardo Reinoso | National Premier Soccer League | Flamingo Park | Miami | 2012 |
| Union Omaha | | USL League One | Werner Park | Omaha, Nebraska | 2019 |
| Tormenta FC | Ian Cameron | USL League One | Erk Russell Park | Statesboro | 2015 |
| Jacksonville Armada | | National Premier Soccer League | Baseball Grounds of Jacksonville | Jacksonville | 2013 |
| Chattanooga FC | | | Finley Stadium | Chattanooga | 2009 |
| Loudoun United | | USL Championship | Loudoun United Stadium | Leesburg | 2018 |
